calculator · mg · mcg · u-100

Peptide calculator

To calculate a peptide dose in units, divide the mg in the vial by the mL of liquid added, divide the amount you need by that concentration, and multiply by 100. Example: 100 mcg from a 5 mg vial mixed with 2 mL is 4 units on a U-100 syringe.

Open Peptide Calculator editorial teamUpdated 11 sources

peptide_calculator

vial + diluent → units on a U-100 syringe

mg
mL
Syringe size (U-100)

Calculating…

Formula. Concentration = mg in vial ÷ mL added. Units = (dose ÷ concentration) × 100 on a U-100 syringe. This tool does math only and does not recommend doses.

Math onlyThis is an independent calculator. It turns the numbers you type into a syringe reading and nothing more. Most peptides discussed online are not FDA-approved for any use, and this site never suggests an amount. Each peptide page states the regulatory status and the evidence, with sources.

How the peptide calculator works

Three numbers decide the answer: how much peptide is in the vial, how much liquid you added, and how much you need to measure. The calculator runs the same three lines of arithmetic you could do on paper.

# 1. concentration of the mixed vialmg_per_mL = vial_mg ÷ diluent_mL# 2. volume that holds the amountmL = (amount_mcg ÷ 1000) ÷ mg_per_mL# 3. U-100 syringe readingunits = mL × 100

Worked example. A 5 mg vial mixed with 2 mL holds 5 ÷ 2 = 2.5 mg/mL. An amount of 100 mcg is 0.1 mg, so it sits in 0.1 ÷ 2.5 = 0.04 mL. On a U-100 syringe that is 0.04 × 100 = 4 units. Each unit of this mix holds 25 mcg.

A U-100 insulin syringe is marked so that 100 units equal 1 mL.[2] If your syringe is marked U-40 or only in mL, the unit numbers on this site do not apply. The full code behind the calculator, with its tests, is on the formulas page.

mcg or mg: check before you type

Peptide amounts are often written in micrograms (mcg), while vials are labeled in milligrams (mg). One milligram is 1,000 micrograms, so 250 mcg is 0.25 mg. The calculator has a mg/mcg switch next to the amount. A slip between the two is a thousandfold error. The mg to mcg chart lists the common conversions.

Concentration quick reference

The concentration depends only on the vial and the diluent volume. These rows cover round combinations. "mcg per unit" tells you what one line on a U-100 syringe holds.

Concentration, mcg per unit and units per 100 mcg
Vial + diluentmg/mLmcg per unitUnits per 100 mcg
2 mg + 1 mL2205
2 mg + 2 mL11010
2 mg + 3 mL0.6676.6715
5 mg + 1 mL5502
5 mg + 2 mL2.5254
5 mg + 3 mL1.66716.676
10 mg + 1 mL101001
10 mg + 2 mL5502
10 mg + 3 mL3.33333.333

Units for example amounts

The grid below is pure arithmetic for a 5 mg vial. The amounts are round numbers chosen to show the pattern; they are not doses for any peptide. For other vial sizes, see the units charts.

5 mg vial: units on a U-100 syringe, by diluent volume
Amount1 mL2 mL2.5 mL3 mL
50 mcg122.53
100 mcg2456
200 mcg481012
250 mcg51012.515
500 mcg10202530
1,000 mcg20405060

Look along any row: doubling the water doubles the units for the same amount. At 1 mL, 50 mcg is only 1 unit, which is below what most syringe markings can show reliably. That is why the diluent volume matters, and it is covered in the reconstitution guide.

Peptide molecular weight from a sequence

A peptide's molecular weight is the sum of its amino acid residues plus one molecule of water: BPC-157's 15 residues come to 1,419.56 g/mol. Paste any sequence in one-letter codes below to get the weight, the formula and the mg-to-nanomole conversion.

peptide_mw

sequence → formula, average and monoisotopic mass

Modifications

Calculating…

Formula. MW = sum of residue masses + H2O. Average masses use IUPAC atomic weights; monoisotopic masses use NIST isotope masses.

How the molecular weight is calculated

Each amino acid in a chain has lost one water molecule to the bond on either side, so the calculator adds up the residue formulas and then adds one water back for the two free ends.

# average molecular weight (g/mol)MW = Σ residue formulas + H2O, using C 12.011, H 1.008, N 14.007, O 15.999, S 32.06# modificationsN-terminal acetyl: + C2H2O C-terminal amide: − O + N + H each disulfide: − 2 H

Atomic weights are the IUPAC abridged standard values,[4] and monoisotopic masses use NIST's isotope masses for 1H, 12C, 14N, 16O and 32S.[5] The code is checked against published formulas every time the site is built:

Calculator output vs published values
PeptideCalculated formulaCalculated MWPublished MW
BPC-157C62H98N16O221,419.561,419.5[6]
TB-500 (Ac-LKKTETQ)C38H68N10O14889.02889[7]
MOTS-cC101H152N28O22S22,174.622,174.6[8]
Sermorelin (C-terminal amide)C149H246N44O42S3,357.933,357.9[9]
CJC-1295, no DAC (amide)C152H252N44O423,367.953,367.95[10]
AOD-9604 (1 disulfide)C78H123N23O23S21,815.11,815.1[11]

Molecular weight is what turns micrograms into molecules. Some studies report amounts in nanomoles per kilogram; ipamorelin's intravenous study is one. Each peptide page shows micrograms and nanomoles side by side.

Peptide pages

Each page has the calculator set up for that peptide's units, its FDA status, and what published human studies show. A page lists doses only when they come from an FDA-approved label or a published human trial.

Guides and reference

What this site will not do

This site does not suggest amounts, link to sellers or hide its math.

  • Suggest amounts. You type the amount. The site does not recommend one, and it publishes no protocols, cycles or "stacks".
  • Link to sellers. There are no shop links, discount codes or vendor reviews anywhere on the site.
  • Hide the math. Every formula is printed next to the tool that uses it.

This matters because of how peptide calculators have been used. In August 2026 the FDA warned a seller of "research use only" peptides. The agency said that selling bacteriostatic water next to a "peptide guide" and a "peptide calculator" showed the products were meant for people.[1] This site sells nothing. It is an arithmetic and education tool, and the formulas are open so anyone can check them.

New to the subject? Start with what peptides are and keep the peptide glossary open for the terms. A peptide drug is a chain of up to 40 amino acids; longer chains are regulated as biological products in the U.S.[3]

Frequently asked questions

How do I calculate a peptide dose in units?

Divide the mg in the vial by the mL of liquid you added to get mg per mL. Divide the amount you need (in mg) by that concentration to get mL. Multiply mL by 100 for units on a U-100 syringe. The calculator above does all three steps.

How many units is 100 mcg?

It depends on the concentration. At 2.5 mg/mL (5 mg in 2 mL) it is 4 units. At 5 mg/mL (5 mg in 1 mL) it is 2 units. At 1 mg/mL (2 mg in 2 mL) it is 10 units.

How much water should I add to a peptide vial?

There is no single number. More water makes small amounts easier to measure but makes larger amounts take more of the syringe. The reconstitution guide shows the trade-off. For an approved medicine, follow its label; for example, the tesamorelin label specifies its own diluent and volume.

Is there a free peptide calculator app?

This page is a free peptide calculator that works in any phone or desktop browser, with nothing to install and no account. Your numbers stay in the browser; the copy-link button saves a result as a URL.

Does the calculator work for every peptide?

Yes. The arithmetic is the same for any powder dissolved in a known volume and measured on a U-100 syringe. The calculator cannot know whether a product actually contains what its label says.

Why does the calculator not suggest a dose?

Because most of these peptides are not approved medicines, and dosing is a medical decision. The site does math and education only. Peptide pages report doses only from approved labels or published human studies, with sources.

Sources

  1. Warning letter: Royal Peptides LLC · U.S. Food and Drug Administration, August 24, 2026
  2. 510(k) clearance K233794, Insulin Syringe ("U-100 means there are 100 units of insulin in every mL") · U.S. Food and Drug Administration, March 6, 2024
  3. Final rule: Definition of the term "biological product" (85 FR 10057) · U.S. Food and Drug Administration, Federal Register, February 21, 2020
  4. Abridged standard atomic weights 2024 · IUPAC Commission on Isotopic Abundances and Atomic Weights (CIAAW), 2024
  5. Atomic weights and isotopic compositions for all elements · National Institute of Standards and Technology, accessed November 13, 2026
  6. PubChem CID 9941957: BPC-157 · PubChem, NCBI, accessed November 13, 2026
  7. PubChem CID 62707662: TB-500 (Ac-LKKTETQ) · PubChem, NCBI, accessed November 13, 2026
  8. PubChem CID 146675088: MOTS-c · PubChem, NCBI, accessed November 13, 2026
  9. PubChem CID 16132413: sermorelin · PubChem, NCBI, accessed November 13, 2026
  10. FDA briefing document: CJC-1295-related bulk drug substances · U.S. Food and Drug Administration, Pharmacy Compounding Advisory Committee, December 4, 2024
  11. PubChem CID 71300630: AOD-9604 · PubChem, NCBI, accessed November 13, 2026